Abstract
Pseudocercospora puerariicola, a leaf spot fungus of Pueraria species, has been reported in East Asian countries, including Korea. Despite the ecological significance of this fungus, to date, no living cultures are present in Korea. The DNA nucleotide sequences of the internal transcribed spacer (ITS) region and the 28S rDNA gene (large subunit, LSU) of the fungus were deposited on GenBank database from Chinese, Laos, and Taiwanese isolates. However, these results were not sufficient to confirm pathogen identification to the species level. Therefore, we aimed to provide further information on this species based on the Korean isolates obtained from Pueraria montana var. lobata, adding more genetic locus to our study to support and affirm the identification of the fungus based on multi-loci analyses from Korean isolates. Nine samples were collected from different areas of Korea and deposited at the Korea University Herbarium, then used in this study. Three monoconidial isolates were obtained in 2022, and molecular phylogenetic analyses were performed using a combined multi-locus dataset of the ITS region, partial actin (actA), partial translation elongation factor 1-alpha (tef-1), and partial DNA-directed RNA polymerase II second largest subunit (rpb2) genes. The present study confirmed the presence of P. puerariicola in Korea as a causal agent of leaf spots on kudzu plants, providing the phylogenetic position of this fungus for the first time based on a multigene dataset and preserving three isolates in an authentic culture collection for future studies.
Original language | English |
---|---|
Journal | Journal of Plant Pathology |
DOIs | |
Publication status | Accepted/In press - 2024 |
Bibliographical note
Publisher Copyright:
© The Author(s) under exclusive licence to Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2024.
Keywords
- Biocontrol
- East Asian arrowroot
- Kudzu
- Multi-locus DNA dataset
- Pseudocercospora puerariae
ASJC Scopus subject areas
- Plant Science
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Choi, I. Y., Abasova, L., Park, J. H. (Accepted/In press). Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea. Journal of Plant Pathology. https://doi.org/10.1007/s42161-024-01749-2
Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea. / Choi, In Young; Abasova, Lamiya; Park, Ji Hyun et al.
In: Journal of Plant Pathology, 2024.
Research output: Contribution to journal › Article › peer-review
Choi, IY, Abasova, L, Park, JH 2024, 'Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea', Journal of Plant Pathology. https://doi.org/10.1007/s42161-024-01749-2
Choi IY, Abasova L, Park JH, Shin HD. Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea. Journal of Plant Pathology. 2024. doi: 10.1007/s42161-024-01749-2
Choi, In Young ; Abasova, Lamiya ; Park, Ji Hyun et al. / Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea. In: Journal of Plant Pathology. 2024.
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abstract = "Pseudocercospora puerariicola, a leaf spot fungus of Pueraria species, has been reported in East Asian countries, including Korea. Despite the ecological significance of this fungus, to date, no living cultures are present in Korea. The DNA nucleotide sequences of the internal transcribed spacer (ITS) region and the 28S rDNA gene (large subunit, LSU) of the fungus were deposited on GenBank database from Chinese, Laos, and Taiwanese isolates. However, these results were not sufficient to confirm pathogen identification to the species level. Therefore, we aimed to provide further information on this species based on the Korean isolates obtained from Pueraria montana var. lobata, adding more genetic locus to our study to support and affirm the identification of the fungus based on multi-loci analyses from Korean isolates. Nine samples were collected from different areas of Korea and deposited at the Korea University Herbarium, then used in this study. Three monoconidial isolates were obtained in 2022, and molecular phylogenetic analyses were performed using a combined multi-locus dataset of the ITS region, partial actin (actA), partial translation elongation factor 1-alpha (tef-1), and partial DNA-directed RNA polymerase II second largest subunit (rpb2) genes. The present study confirmed the presence of P. puerariicola in Korea as a causal agent of leaf spots on kudzu plants, providing the phylogenetic position of this fungus for the first time based on a multigene dataset and preserving three isolates in an authentic culture collection for future studies.",
keywords = "Biocontrol, East Asian arrowroot, Kudzu, Multi-locus DNA dataset, Pseudocercospora puerariae",
author = "Choi, {In Young} and Lamiya Abasova and Park, {Ji Hyun} and Shin, {Hyeon Dong}",
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AU - Choi, In Young
AU - Abasova, Lamiya
AU - Park, Ji Hyun
AU - Shin, Hyeon Dong
N1 - Publisher Copyright:© The Author(s) under exclusive licence to Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2024.
PY - 2024
Y1 - 2024
N2 - Pseudocercospora puerariicola, a leaf spot fungus of Pueraria species, has been reported in East Asian countries, including Korea. Despite the ecological significance of this fungus, to date, no living cultures are present in Korea. The DNA nucleotide sequences of the internal transcribed spacer (ITS) region and the 28S rDNA gene (large subunit, LSU) of the fungus were deposited on GenBank database from Chinese, Laos, and Taiwanese isolates. However, these results were not sufficient to confirm pathogen identification to the species level. Therefore, we aimed to provide further information on this species based on the Korean isolates obtained from Pueraria montana var. lobata, adding more genetic locus to our study to support and affirm the identification of the fungus based on multi-loci analyses from Korean isolates. Nine samples were collected from different areas of Korea and deposited at the Korea University Herbarium, then used in this study. Three monoconidial isolates were obtained in 2022, and molecular phylogenetic analyses were performed using a combined multi-locus dataset of the ITS region, partial actin (actA), partial translation elongation factor 1-alpha (tef-1), and partial DNA-directed RNA polymerase II second largest subunit (rpb2) genes. The present study confirmed the presence of P. puerariicola in Korea as a causal agent of leaf spots on kudzu plants, providing the phylogenetic position of this fungus for the first time based on a multigene dataset and preserving three isolates in an authentic culture collection for future studies.
AB - Pseudocercospora puerariicola, a leaf spot fungus of Pueraria species, has been reported in East Asian countries, including Korea. Despite the ecological significance of this fungus, to date, no living cultures are present in Korea. The DNA nucleotide sequences of the internal transcribed spacer (ITS) region and the 28S rDNA gene (large subunit, LSU) of the fungus were deposited on GenBank database from Chinese, Laos, and Taiwanese isolates. However, these results were not sufficient to confirm pathogen identification to the species level. Therefore, we aimed to provide further information on this species based on the Korean isolates obtained from Pueraria montana var. lobata, adding more genetic locus to our study to support and affirm the identification of the fungus based on multi-loci analyses from Korean isolates. Nine samples were collected from different areas of Korea and deposited at the Korea University Herbarium, then used in this study. Three monoconidial isolates were obtained in 2022, and molecular phylogenetic analyses were performed using a combined multi-locus dataset of the ITS region, partial actin (actA), partial translation elongation factor 1-alpha (tef-1), and partial DNA-directed RNA polymerase II second largest subunit (rpb2) genes. The present study confirmed the presence of P. puerariicola in Korea as a causal agent of leaf spots on kudzu plants, providing the phylogenetic position of this fungus for the first time based on a multigene dataset and preserving three isolates in an authentic culture collection for future studies.
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