Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea (2025)

Abstract

Pseudocercospora puerariicola, a leaf spot fungus of Pueraria species, has been reported in East Asian countries, including Korea. Despite the ecological significance of this fungus, to date, no living cultures are present in Korea. The DNA nucleotide sequences of the internal transcribed spacer (ITS) region and the 28S rDNA gene (large subunit, LSU) of the fungus were deposited on GenBank database from Chinese, Laos, and Taiwanese isolates. However, these results were not sufficient to confirm pathogen identification to the species level. Therefore, we aimed to provide further information on this species based on the Korean isolates obtained from Pueraria montana var. lobata, adding more genetic locus to our study to support and affirm the identification of the fungus based on multi-loci analyses from Korean isolates. Nine samples were collected from different areas of Korea and deposited at the Korea University Herbarium, then used in this study. Three monoconidial isolates were obtained in 2022, and molecular phylogenetic analyses were performed using a combined multi-locus dataset of the ITS region, partial actin (actA), partial translation elongation factor 1-alpha (tef-1), and partial DNA-directed RNA polymerase II second largest subunit (rpb2) genes. The present study confirmed the presence of P. puerariicola in Korea as a causal agent of leaf spots on kudzu plants, providing the phylogenetic position of this fungus for the first time based on a multigene dataset and preserving three isolates in an authentic culture collection for future studies.

Original languageEnglish
JournalJournal of Plant Pathology
DOIs
Publication statusAccepted/In press - 2024

Bibliographical note

Publisher Copyright:
© The Author(s) under exclusive licence to Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2024.

Keywords

  • Biocontrol
  • East Asian arrowroot
  • Kudzu
  • Multi-locus DNA dataset
  • Pseudocercospora puerariae

ASJC Scopus subject areas

  • Plant Science

Access to Document

Other files and links

Fingerprint

Dive into the research topics of 'Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea'. Together they form a unique fingerprint.

View full fingerprint

Cite this

  • APA
  • Standard
  • Harvard
  • Vancouver
  • Author
  • BIBTEX
  • RIS

Choi, I. Y., Abasova, L., Park, J. H. (Accepted/In press). Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea. Journal of Plant Pathology. https://doi.org/10.1007/s42161-024-01749-2

Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea. / Choi, In Young; Abasova, Lamiya; Park, Ji Hyun et al.
In: Journal of Plant Pathology, 2024.

Research output: Contribution to journalArticlepeer-review

Choi, IY, Abasova, L, Park, JH 2024, 'Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea', Journal of Plant Pathology. https://doi.org/10.1007/s42161-024-01749-2

Choi IY, Abasova L, Park JH, Shin HD. Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea. Journal of Plant Pathology. 2024. doi: 10.1007/s42161-024-01749-2

Choi, In Young ; Abasova, Lamiya ; Park, Ji Hyun et al. / Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea. In: Journal of Plant Pathology. 2024.

@article{07c119bff4014c1d88dbac8d218fc54c,

title = "Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea",

abstract = "Pseudocercospora puerariicola, a leaf spot fungus of Pueraria species, has been reported in East Asian countries, including Korea. Despite the ecological significance of this fungus, to date, no living cultures are present in Korea. The DNA nucleotide sequences of the internal transcribed spacer (ITS) region and the 28S rDNA gene (large subunit, LSU) of the fungus were deposited on GenBank database from Chinese, Laos, and Taiwanese isolates. However, these results were not sufficient to confirm pathogen identification to the species level. Therefore, we aimed to provide further information on this species based on the Korean isolates obtained from Pueraria montana var. lobata, adding more genetic locus to our study to support and affirm the identification of the fungus based on multi-loci analyses from Korean isolates. Nine samples were collected from different areas of Korea and deposited at the Korea University Herbarium, then used in this study. Three monoconidial isolates were obtained in 2022, and molecular phylogenetic analyses were performed using a combined multi-locus dataset of the ITS region, partial actin (actA), partial translation elongation factor 1-alpha (tef-1), and partial DNA-directed RNA polymerase II second largest subunit (rpb2) genes. The present study confirmed the presence of P. puerariicola in Korea as a causal agent of leaf spots on kudzu plants, providing the phylogenetic position of this fungus for the first time based on a multigene dataset and preserving three isolates in an authentic culture collection for future studies.",

keywords = "Biocontrol, East Asian arrowroot, Kudzu, Multi-locus DNA dataset, Pseudocercospora puerariae",

author = "Choi, {In Young} and Lamiya Abasova and Park, {Ji Hyun} and Shin, {Hyeon Dong}",

note = "Publisher Copyright: {\textcopyright} The Author(s) under exclusive licence to Societ{\`a} Italiana di Patologia Vegetale (S.I.Pa.V.) 2024.",

year = "2024",

doi = "10.1007/s42161-024-01749-2",

language = "English",

journal = "Journal of Plant Pathology",

issn = "1125-4653",

publisher = "Springer Science and Business Media Deutschland GmbH",

}

TY - JOUR

T1 - Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea

AU - Choi, In Young

AU - Abasova, Lamiya

AU - Park, Ji Hyun

AU - Shin, Hyeon Dong

N1 - Publisher Copyright:© The Author(s) under exclusive licence to Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2024.

PY - 2024

Y1 - 2024

N2 - Pseudocercospora puerariicola, a leaf spot fungus of Pueraria species, has been reported in East Asian countries, including Korea. Despite the ecological significance of this fungus, to date, no living cultures are present in Korea. The DNA nucleotide sequences of the internal transcribed spacer (ITS) region and the 28S rDNA gene (large subunit, LSU) of the fungus were deposited on GenBank database from Chinese, Laos, and Taiwanese isolates. However, these results were not sufficient to confirm pathogen identification to the species level. Therefore, we aimed to provide further information on this species based on the Korean isolates obtained from Pueraria montana var. lobata, adding more genetic locus to our study to support and affirm the identification of the fungus based on multi-loci analyses from Korean isolates. Nine samples were collected from different areas of Korea and deposited at the Korea University Herbarium, then used in this study. Three monoconidial isolates were obtained in 2022, and molecular phylogenetic analyses were performed using a combined multi-locus dataset of the ITS region, partial actin (actA), partial translation elongation factor 1-alpha (tef-1), and partial DNA-directed RNA polymerase II second largest subunit (rpb2) genes. The present study confirmed the presence of P. puerariicola in Korea as a causal agent of leaf spots on kudzu plants, providing the phylogenetic position of this fungus for the first time based on a multigene dataset and preserving three isolates in an authentic culture collection for future studies.

AB - Pseudocercospora puerariicola, a leaf spot fungus of Pueraria species, has been reported in East Asian countries, including Korea. Despite the ecological significance of this fungus, to date, no living cultures are present in Korea. The DNA nucleotide sequences of the internal transcribed spacer (ITS) region and the 28S rDNA gene (large subunit, LSU) of the fungus were deposited on GenBank database from Chinese, Laos, and Taiwanese isolates. However, these results were not sufficient to confirm pathogen identification to the species level. Therefore, we aimed to provide further information on this species based on the Korean isolates obtained from Pueraria montana var. lobata, adding more genetic locus to our study to support and affirm the identification of the fungus based on multi-loci analyses from Korean isolates. Nine samples were collected from different areas of Korea and deposited at the Korea University Herbarium, then used in this study. Three monoconidial isolates were obtained in 2022, and molecular phylogenetic analyses were performed using a combined multi-locus dataset of the ITS region, partial actin (actA), partial translation elongation factor 1-alpha (tef-1), and partial DNA-directed RNA polymerase II second largest subunit (rpb2) genes. The present study confirmed the presence of P. puerariicola in Korea as a causal agent of leaf spots on kudzu plants, providing the phylogenetic position of this fungus for the first time based on a multigene dataset and preserving three isolates in an authentic culture collection for future studies.

KW - Biocontrol

KW - East Asian arrowroot

KW - Kudzu

KW - Multi-locus DNA dataset

KW - Pseudocercospora puerariae

UR - http://www.scopus.com/inward/record.url?scp=85201939157&partnerID=8YFLogxK

U2 - 10.1007/s42161-024-01749-2

DO - 10.1007/s42161-024-01749-2

M3 - Article

AN - SCOPUS:85201939157

SN - 1125-4653

JO - Journal of Plant Pathology

JF - Journal of Plant Pathology

ER -

Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea (2025)
Top Articles
Latest Posts
Recommended Articles
Article information

Author: Reed Wilderman

Last Updated:

Views: 6674

Rating: 4.1 / 5 (52 voted)

Reviews: 91% of readers found this page helpful

Author information

Name: Reed Wilderman

Birthday: 1992-06-14

Address: 998 Estell Village, Lake Oscarberg, SD 48713-6877

Phone: +21813267449721

Job: Technology Engineer

Hobby: Swimming, Do it yourself, Beekeeping, Lapidary, Cosplaying, Hiking, Graffiti

Introduction: My name is Reed Wilderman, I am a faithful, bright, lucky, adventurous, lively, rich, vast person who loves writing and wants to share my knowledge and understanding with you.